For image tiles without focus correction points, the software will calculate the focuses based on other focus points. Move the stage to a position, adjust focus, and add ('+') the position (X, Y, and Z) in memory (step 6) Repeat adding focus correction points You don't have to set focus for all tiles. Set focus correction points ('Focus Surface'): this function allows the program to use focuses determined by the user to counteract the uneven surface of the tissues section.Change to objective you wish to use and set focus correction points (see below).rectangle, oval, free-drawing) (optional step 2) and draw a region in the previewed image The newly marked area will show up as a new tile region in the region list. In 'Tile Region Setup', choose a desired 'Contour' (e.g.Set up a region (see above) using a low magnification (e.g.
(Optional) Tile region setup following a preview scan:.You can also drag the yellow line with the mouse to resize the area (expanding or trimming) (step 5 ). Moving the stage to a position outside the current region (red lined) and adding 'stake' again will expand the boundary.The actual area that the final image covers shows up as red colored tiles. To mark region by 'Stakes', move the stage manually using the rotary controller or by double-clicking in the virtual stage panel in ZEN, add stakes (step 4) You need to put down at least two 'stakes' for the software to mark an area around them (marked with yellow borders).To mark region by 'Tiles' simply put numbers in row and column, and click plus sign (+).Mark the area to be imaged ('Tile Regions'):.The center screen area is split into two regions: a vitual stage on the left and a live image of the current field on the right. Open 'Advanced Setup' (step 2) and the 'Live' function starts.Open the functional module 'Tiles' (step 1).Set up exposure for all channels as described above.
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